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Obtaining a venous blood sample for determination of basal blood glucose basal blood glucose

Removal of a venous blood sample by percutaneous puncture.


– To measure and determine blood glucose values. – To assess the efficacy of insulin administration.


– Trough. – Blood glucose measuring device. – Sharps container.


– Sterile gauze. – Non-sterile gloves. – Antiseptic solution. – Lancing device. – Blood glucose test strip. – Absorbent cotton. – Nursing records.


– Perform hand washing. – Prepare the material. – Preserve the patient’s privacy.

– Verify the correct identification of the patient. – Inform the patient of the procedure. – Ask the patient to cooperate and remain still during the puncture. – Place the patient in a suitable position. – Check the patient’s hand hygiene. – Check that the glucose meter is working properly and insert a strip into it. – Perform a light massage of the selected area to promote circulation. – Disinfect the area with absorbent cotton soaked in antiseptic solution. – Allow to dry. – Put on non-sterile gloves. – Select the degree of depth according to the needs of the patient’s skin. – Puncture the lateral area of the finger with the lancet, discard the first drop and apply the drop of blood on the test strip. – The trajectory of the puncture should be straight. – Press the finger with the cotton. – Discard needle and sharps in the container, according to waste segregation criteria. – Place the patient in a comfortable position. – Collect the material. – Remove gloves. – Wash hands. – Record in nursing documentation: procedure, reason, date and time, blood glucose values, incidences and patient response.


– Do not use alcohol since it hardens the puncture site, causes stinging and may give an erroneous value. – In infants and neonates, the sample should be obtained from the heel. – Notify the physician if glucose levels are abnormal. – Do not puncture cold, cyanotic or edematous areas.

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